Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cardiomyocyte T-tubule loss in heart failure - NFκB as a central signalling hub.

Cardiovascular research·2026
Same author

Development of a Nanoscale Protein-Protein Mapping of PDE4 Interface-Disrupting Peptides.

Nano letters·2026
Same author

Decreasing Microtubule Detyrosination Improves Cardiac Mechanics and Sodium Channel Function in Arrhythmogenic Cardiomyopathy.

Circulation. Arrhythmia and electrophysiology·2026
Same author

Profiling Protein Aggregate Size Using Single-Molecule Array Technology.

Analytical chemistry·2026
Same author

Loss of caveolar A1 adenosine receptor signalling blunts anti-adrenergic control in heart failure.

Cardiovascular research·2026
Same author

Divergent toxicity mechanisms of amyloid-beta aggregates arising from a single aggregation reaction.

Cell reports·2026

Related Experiment Video

Updated: May 2, 2026

Detection of Fluorescent Nanoparticle Interactions with Primary Immune Cell Subpopulations by Flow Cytometry
07:31

Detection of Fluorescent Nanoparticle Interactions with Primary Immune Cell Subpopulations by Flow Cytometry

Published on: March 28, 2014

19.1K

Imaging single nanoparticle interactions with human lung cells using fast ion conductance microscopy.

Pavel Novak1, Andrew Shevchuk, Pakatip Ruenraroengsak

  • 1School of Engineering and Materials Science, Queen Mary University of London , Mile End Rd, London E1 4NS, United Kingdom.

Nano Letters
|February 22, 2014
PubMed
Summary

New high-speed imaging reveals dynamic nanoparticle-membrane interactions at the nanoscale. This breakthrough offers crucial insights into nanoparticle behavior for biomedical applications.

More Related Videos

Long-term High-Resolution Intravital Microscopy in the Lung with a Vacuum Stabilized Imaging Window
07:19

Long-term High-Resolution Intravital Microscopy in the Lung with a Vacuum Stabilized Imaging Window

Published on: October 6, 2016

12.8K
Advanced Imaging of Lung Homing Human Lymphocytes in an Experimental In Vivo Model of Allergic Inflammation Based on Light-sheet Microscopy
10:39

Advanced Imaging of Lung Homing Human Lymphocytes in an Experimental In Vivo Model of Allergic Inflammation Based on Light-sheet Microscopy

Published on: April 16, 2019

7.0K

Related Experiment Videos

Last Updated: May 2, 2026

Detection of Fluorescent Nanoparticle Interactions with Primary Immune Cell Subpopulations by Flow Cytometry
07:31

Detection of Fluorescent Nanoparticle Interactions with Primary Immune Cell Subpopulations by Flow Cytometry

Published on: March 28, 2014

19.1K
Long-term High-Resolution Intravital Microscopy in the Lung with a Vacuum Stabilized Imaging Window
07:19

Long-term High-Resolution Intravital Microscopy in the Lung with a Vacuum Stabilized Imaging Window

Published on: October 6, 2016

12.8K
Advanced Imaging of Lung Homing Human Lymphocytes in an Experimental In Vivo Model of Allergic Inflammation Based on Light-sheet Microscopy
10:39

Advanced Imaging of Lung Homing Human Lymphocytes in an Experimental In Vivo Model of Allergic Inflammation Based on Light-sheet Microscopy

Published on: April 16, 2019

7.0K

Area of Science:

  • Nanotechnology
  • Biomedical Engineering
  • Cell Biology

Background:

  • Understanding nanoparticle-cell interactions is vital for developing effective nanomedicines.
  • Current imaging techniques lack the resolution and speed to capture dynamic nanoscale events.
  • Experimental data on nanoparticle-membrane interactions remain limited.

Purpose of the Study:

  • To develop and demonstrate a novel imaging approach for observing dynamic interactions between individual nanoparticles and cell membranes.
  • To overcome the limitations of existing imaging techniques in studying nanoscale biological processes.

Main Methods:

  • Utilized a modified scanning ion conductance microscope (SICM).
  • Enabled simultaneous high-speed topographical and fluorescence imaging.
  • Tracked single 200 nm carboxyl-modified nanoparticles interacting with membrane structures at 15 frames per second.

Main Results:

  • Successfully visualized dynamic interactions between individual nanoparticles and membrane structures in real-time.
  • Achieved high-speed imaging at 15 s/frame, providing unprecedented temporal resolution.
  • Demonstrated the capability to identify specific membrane structures during nanoparticle interactions.

Conclusions:

  • The developed imaging approach provides a new window into the complexity of nanoparticle-cell interactions.
  • This technique is essential for advancing the biomedical applications of nanoparticles.
  • Further research can leverage this method to explore nanoparticle behavior in various biological contexts.