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Immortalization of rat keratinocytes by transfection with polyomavirus large T gene
S A Bayley1, A J Stones, C G Smith
1Biosciences Division, Unilever Research, Sharnbrook, Bedford, United Kingdom.
Experimental Cell Research
|July 1, 1988
Abstract:
Transfection of primary rat keratinocytes with the polyomavirus large T gene promotes the establishment of cell lines. The keratinocytes express the large T protein and can be continuously cultured in medium containing a low concentration of calcium. The immortalized keratinocytes retain the ability to differentiate when the calcium concentration is increased to normal levels.