Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mouse Models of Cancer Study02:43

Mouse Models of Cancer Study

4.7K
Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
4.7K
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

14.8K
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
14.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Olfactory perception, odor interaction and aroma intensity prediction of terpenes in wine beverage.

NPJ science of food·2026
Same author

MRI-based quantification of intratumoral heterogeneity for differentiating glioblastoma from solitary brain metastasis: a two-center study.

European journal of radiology·2026
Same author

Physical Activity and Social Anxiety in Highly Sensitive Individuals: The Mediating Role of Cognitive Reappraisal.

Psychological reports·2026
Same author

Intravenous lidocaine reduces propofol requirements during endoscopic ultrasound: a randomized, double-blinded, controlled trial.

BMC anesthesiology·2026
Same author

Mechanisms of Microglial NLRP3 Inflammasome Activation by Methylmalonic Acid in Methylmalonic Acidemia.

Mediators of inflammation·2026
Same author

Severe Methotrexate toxicity after ectopic pregnancy: a case report managed with continuous renal replacement therapy.

Cancer chemotherapy and pharmacology·2026

Related Experiment Video

Updated: May 2, 2026

A Simple and Inexpensive Method for Determining Cold Sensitivity and Adaptation in Mice
08:35

A Simple and Inexpensive Method for Determining Cold Sensitivity and Adaptation in Mice

Published on: March 17, 2015

14.8K

Mice lacking G0S2 are lean and cold-tolerant.

Tian Ma1, Alexandra G N Lopez-Aguiar1, Aihua Li2

  • 1Department of Pharmacology and Toxicology; Geisel School of Medicine at Dartmouth; Hanover, NH USA.

Cancer Biology & Therapy
|February 22, 2014
PubMed
Summary

Mice lacking G0S2 (G0S2-/-) showed impaired lactation and altered energy balance, with decreased weight gain and improved cold tolerance. G0S2 protein is crucial for thermogenesis and lipolysis.

Keywords:
G0S2body weightcold toleranceknockout micelactationmetabolismtumor suppressor

More Related Videos

Assessment of the Metabolic Effects of Isocaloric 2:1 Intermittent Fasting in Mice
08:06

Assessment of the Metabolic Effects of Isocaloric 2:1 Intermittent Fasting in Mice

Published on: November 27, 2019

8.7K
Assessment of Sexual Behavior of Male Mice
04:38

Assessment of Sexual Behavior of Male Mice

Published on: March 5, 2020

20.4K

Related Experiment Videos

Last Updated: May 2, 2026

A Simple and Inexpensive Method for Determining Cold Sensitivity and Adaptation in Mice
08:35

A Simple and Inexpensive Method for Determining Cold Sensitivity and Adaptation in Mice

Published on: March 17, 2015

14.8K
Assessment of the Metabolic Effects of Isocaloric 2:1 Intermittent Fasting in Mice
08:06

Assessment of the Metabolic Effects of Isocaloric 2:1 Intermittent Fasting in Mice

Published on: November 27, 2019

8.7K
Assessment of Sexual Behavior of Male Mice
04:38

Assessment of Sexual Behavior of Male Mice

Published on: March 5, 2020

20.4K

Area of Science:

  • Biochemistry
  • Physiology
  • Genetics

Background:

  • G0S2 (G 0/G 1 switch gene 2) is a protein with suggested roles in tumor suppression, immune regulation, and lipolysis.
  • Its direct involvement in cell cycle regulation remains unclear.
  • Previous research indicated potential functions beyond its initial identification.

Purpose of the Study:

  • To definitively characterize the in vivo functions of G0S2.
  • To investigate the physiological roles of G0S2 using genetically modified mice.
  • To explore the impact of G0S2 absence on metabolic processes and thermoregulation.

Main Methods:

  • Generation and characterization of G0S2 knockout (G0S2-/-) mice.
  • Phenotypic analysis including body weight, fat pad weight, serum glycerol levels, food intake, activity, and cold tolerance.
  • Assessment of lactation in G0S2-/- female mice.
  • Gene expression analysis related to thermogenesis.

Main Results:

  • G0S2-/- mice exhibited normal development but had a significant lactation defect.
  • These mice showed decreased body weight gain, reduced gonadal fat pad weight, and increased serum glycerol.
  • G0S2 deficiency improved acute cold tolerance and augmented thermogenesis gene expression.
  • Diet-induced obesity was not prevented by G0S2 loss.

Conclusions:

  • G0S2 plays critical roles in lactation, energy balance, and thermogenesis.
  • The findings support a role for G0S2 in regulating lipolysis, potentially explaining its tumor suppressive effects.
  • G0S2 is a key regulator of metabolic homeostasis and adaptive responses to cold.