Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Improved avidin-biotin-peroxidase complex (ABC) staining.

G Cattoretti1, E Berti, R Schiró

  • 1Division of Anatomic Pathology and Cytology, Istituto Nazionale per lo Studio e la Cura dei Tumori, Milano, Italy.

The Histochemical Journal
|February 1, 1988
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Precision Spectral Measurements of Chromium and Titanium from 10 to 250  GeV/n and Sub-Iron to Iron Ratio with the Calorimetric Electron Telescope on the International Space Station.

Physical review letters·2025
Same author

Classifications of cutaneous lymphomas and lymphoproliferative disorders: An update from the EORTC cutaneous lymphoma histopathology group.

Journal of the European Academy of Dermatology and Venereology : JEADV·2024
Same author

The unexpected guest: Cytotoxic, pseudolymphoma-like reaction at the site of primary cutaneous follicle centre B-cell lymphoma in a patient receiving secukinumab for psoriasis.

Journal of the European Academy of Dermatology and Venereology : JEADV·2024
Same author

Clinicopathological definition, management and prognostic value of mogamulizumab-associated rash and other cutaneous events: A systematic review.

Journal of the European Academy of Dermatology and Venereology : JEADV·2024
Same author

Direct Measurement of the Spectral Structure of Cosmic-Ray Electrons+Positrons in the TeV Region with CALET on the International Space Station.

Physical review letters·2023
Same author

Erratum: Charge-Sign Dependent Cosmic-Ray Modulation Observed with the Calorimetric Electron Telescope on the International Space Station [Phys. Rev. Lett. 130, 211001 (2023)].

Physical review letters·2023

This study enhances the avidin-biotin-peroxidase complex (ABC) staining method by sequential complex addition. This improves antigen detection sensitivity, especially for masked antigens in paraffin sections.

Area of Science:

  • Immunohistochemistry
  • Biochemistry

Background:

  • The avidin-biotin-peroxidase complex (ABC) staining system is widely used in immunohistochemistry.
  • Enhancing the sensitivity of ABC staining is crucial for detecting low-abundance antigens.

Purpose of the Study:

  • To develop a method for intensifying the avidin-biotin-peroxidase complex (ABC) staining system.
  • To improve the detection of antigens, particularly those masked by fixation processes.

Main Methods:

  • Sequential application of an avidin-rich complex followed by a biotin-rich complex to immunostained sections.
  • Each step facilitates horseradish peroxidase deposition and complementary complex binding.

Main Results:

  • Achieved considerable intensification of the ABC staining system.

Related Experiment Videos

  • Enabled the use of higher dilutions of antisera.
  • Demonstrated the ability to detect minute amounts of antigen masked by fixatives on paraffin sections.

    • Conclusions:

    • The sequential complex addition technique significantly enhances ABC staining sensitivity.
    • This method allows for improved visualization of low-concentration or masked antigens in histological samples.