Navigating the cell: how motors overcome roadblocks and traffic jams to efficiently transport cargo

Intracellular transport plays an essential role in maintaining the organization of cells. The importance of long-range, bi-directional transport is evidenced by the fact that its failure goes hand in hand with several diseases including neurodegenerative diseases such as Alzheimer's and Amyotrophic Lateral Sclerosis. The nanoscale cellular transport machinery consisting of cytoskeletal tracks and motor-proteins is responsible for effectively delivering important materials to specific locations inside the cell. Motor-proteins manage to overcome several challenges in the crowded cellular environment to achieve well-coordinated and effective transport. In recent years, thanks to state-of-the-art single molecule biophysical tools, we have started to gain insights into the cellular traffic rules. This perspective summarizes the challenges that motors face in navigating the complex cytoskeleton and the lessons learned about transport in crowded environments from both bottom-up in vitro studies as well as top-down in vivo studies.