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Histamine release from human adenoidal and mesenteric mast cells induced by bacterial antigens
E Brzezińska-Błaszczyk1, M Czuwaj, J Wyczółkowska
1Department of Biogenic Amines, Polish Academy of Sciences, Lódź.
Abstract:
The histamine-releasing capability of Staphylococcus aureus antigens was examined in human adenoidal and mesenteric mast cells obtained by enzymic dispersion of tissues from non-allergic patients. Both populations of mast cells released histamine after challenge with bacterial protein in concentrations between 5-500 micrograms/ml. The release was dependent on the dose, temperature and metabolic energy. The maximum release was observed at 15 min after challenge. The present results suggest that Staphylococcus aureus antigens release histamine from human adenoidal and mesenteric mast cells via a non-cytotoxic, active secretory process.
Insights
Staphylococcus aureus antigens trigger histamine release from human mast cells in the adenoids and mesentery. This non-cytotoxic, active process is dependent on dose, temperature, and metabolic energy.
Area of Science:
- Immunology
- Microbiology
- Cell Biology
Background:
- Mast cells are key immune cells involved in allergic reactions and inflammation.
- Staphylococcus aureus is a common bacterium that can cause various infections.
Purpose of the Study:
- To investigate the effect of Staphylococcus aureus antigens on histamine release from human adenoidal and mesenteric mast cells.
- To determine the mechanism and kinetics of histamine release induced by bacterial antigens.
Main Methods:
- Enzymatic dispersion of human adenoidal and mesenteric tissues to isolate mast cells.
- Challenge of mast cells with varying concentrations of Staphylococcus aureus antigens.
- Measurement of histamine release and assessment of its dependence on dose, temperature, and metabolic energy.
Main Results:
- Staphylococcus aureus antigens induced histamine release from both adenoidal and mesenteric mast cells.
- Histamine release was observed in antigen concentrations ranging from 5-500 µg/ml.
- The release process was dose-dependent, temperature-sensitive, and required metabolic energy, with maximum release at 15 minutes.
- The secretory process was identified as non-cytotoxic.
Conclusions:
- Staphylococcus aureus antigens can actively induce histamine release from human mast cells.
- This histamine release mechanism is non-cytotoxic and involves an active secretory process.
- Findings contribute to understanding the role of bacterial components in mast cell activation and potential inflammatory responses.