Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Real Time RT-PCR02:57

Real Time RT-PCR

51.9K
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
51.9K
RNA-seq03:21

RNA-seq

9.4K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.4K
Ribosome Profiling02:24

Ribosome Profiling

3.2K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Functional and post-translational characterization of pyruvate dehydrogenase demonstrates repression of activity in the liver but not skeletal muscle of the Richardson's ground squirrel (Urocitellus richardsonii) during hibernation.

Journal of thermal biology·2021
Same author

The effect of long-term cold acclimation on redox state and antioxidant defense in the high-altitude frog, Nanorana pleskei.

Journal of thermal biology·2021
Same author

Epigenetic underpinnings of freeze avoidance in the goldenrod gall moth, Epiblema scudderiana.

Journal of insect physiology·2021
Same author

MicroRNA-mediated inhibition of AMPK coordinates tissue-specific downregulation of skeletal muscle metabolism in hypoxic naked mole-rats.

The Journal of experimental biology·2021
Same author

Insight into the Phylogenetic Relationships among Three Subfamilies within Heptageniidae (Insecta: Ephemeroptera) along with Low-Temperature Selection Pressure Analyses Using Mitogenomes.

Insects·2021
Same author

Hypothermia promotes mitochondrial elongation In cardiac cells via inhibition of Drp1.

Cryobiology·2021

Related Experiment Video

Updated: May 2, 2026

Dot Blot Assay for Detecting Global N6-Methyladenosine RNA Modification Levels
08:40

Dot Blot Assay for Detecting Global N6-Methyladenosine RNA Modification Levels

Published on: February 6, 2026

253

A high-throughput protocol for message RNA quantification using RNA dot-blots.

Andrew N Rouble1, Kyle K Biggar1, Kenneth B Storey1

  • 1Institute of Biochemistry and Department of Biology, Carleton University, Ottawa, ON K1S 5B6, Canada.

Analytical Biochemistry
|February 25, 2014
PubMed
Summary
This summary is machine-generated.

This study presents a rapid dot-blotting method to measure messenger RNA (mRNA) levels. The technique effectively quantifies relative mRNA abundance in turtle tissues under varying oxygen conditions.

Keywords:
Anoxia toleranceDot-blot ribonucleotide hybridizationStress-responsive mRNA analysisTurtle

More Related Videos

RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs
14:41

RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs

Published on: July 11, 2020

8.5K
Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing
14:15

Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing

Published on: November 18, 2014

11.4K

Related Experiment Videos

Last Updated: May 2, 2026

Dot Blot Assay for Detecting Global N6-Methyladenosine RNA Modification Levels
08:40

Dot Blot Assay for Detecting Global N6-Methyladenosine RNA Modification Levels

Published on: February 6, 2026

253
RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs
14:41

RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs

Published on: July 11, 2020

8.5K
Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing
14:15

Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing

Published on: November 18, 2014

11.4K

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Physiology

Background:

  • Accurate quantification of messenger RNA (mRNA) is crucial for understanding gene expression.
  • Traditional methods for measuring mRNA abundance can be time-consuming and complex.
  • Developing rapid and simple techniques is essential for comparative analyses.

Purpose of the Study:

  • To develop and validate a rapid dot-blotting method for quantifying relative mRNA abundance in total RNA samples.
  • To assess the utility of this method for comparing mRNA levels across different biological conditions.
  • To demonstrate the application of the technique in a physiological study.

Main Methods:

  • Utilized a dot-blotting technique for RNA analysis.
  • Employed biotin-labeled probes targeting the polyadenylate tail of mRNA for detection.
  • Applied the method to measure relative total mRNA levels in turtle (Trachemys scripta elegans) tissues.

Main Results:

  • The developed dot-blotting method provides a rapid assessment of relative mRNA abundance.
  • Effectively determined differences in total mRNA levels in turtle tissues under normoxic and anoxic conditions.
  • Demonstrated the method's capacity for straightforward comparative analysis.

Conclusions:

  • The biotin-labeled probe dot-blotting technique offers a simple and fast approach for analyzing relative total mRNA levels.
  • This method is valuable for various comparative studies in molecular biology and physiology.
  • The technique is effective for assessing mRNA abundance changes in response to environmental stimuli.