Laccase biosensor based on electrospun copper/carbon composite nanofibers for catechol detection.

Area of Science:

• Materials Science
• Electrochemistry
• Biotechnology

Background:

• Laccase biosensors are crucial for detecting various analytes.
• Carbon nanofibers (CNFs) offer a promising scaffold for biosensor development.
• Enhancing the performance of CNF-based biosensors is an active research area.

Purpose of the Study:

• To compare the biosensing performance of laccase biosensors fabricated with carbon nanofibers (CNFs) and copper/carbon composite nanofibers (Cu/CNFs).
• To investigate the structural and morphological characteristics of CNFs and Cu/CNFs.
• To evaluate the analytical capabilities of Cu/CNF-based laccase biosensors for catechol detection.

Main Methods:

• Electrospinning and carbonization were used to prepare CNFs and Cu/CNFs.
• Scanning electron microscopy (SEM), X-ray diffraction (XRD), and Raman spectroscopy characterized the nanofiber structures.
• Amperometric measurements were performed to assess biosensor performance.

Main Results:

• The Cu/CNFs/laccase/Nafion/glass carbon electrode (GCE) demonstrated superior analytical performance for catechol detection compared to CNFs/laccase/Nafion/GCE.
• The Cu/CNFs-based biosensor exhibited higher sensitivity (33.1 μA/mM), a wider linear range (9.95 × 10⁻⁶ to 9.76 × 10⁻³ M), and a lower detection limit (1.18 μM).
• The Cu/CNFs-based biosensor showed excellent repeatability, reproducibility, selectivity, and long-term stability.

Conclusions:

• Electrospun copper/carbon composite nanofibers significantly enhance the performance of laccase biosensors.
• Cu/CNFs provide a superior platform for developing sensitive and stable catechol biosensors.
• These findings indicate a strong potential for Cu/CNFs in advanced biosensing applications.