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Mouse placental 57-kDa calcium-binding protein: I. Cloning of cDNA and characterization of developmental expression
1Department of Biology, University of Pennsylvania, Philadelphia 19104.
A cDNA clone to the mouse placental 57-kDa calcium-binding protein (MCaBP) [29] was isolated by immunoscreening a mouse placenta cDNA library constructed in the expression phage vector, lambda gt 11. The MCaBP cDNA was 0.7 kb in size, with restriction sites for StuI and Bg/II, and its identity to the MCaBP was confirmed by mRNA hybrid selection. RNA blot hybridization revealed a predominant, 3.9-kb transcript of the MCaBP in day-18 mouse placenta. The expression of the MCaBP during development was analyzed with respect to the levels of protein activity, translatable MCaBP mRNA, and total MCaBP transcript.
A cDNA clone to the mouse placental 57-kDa calcium-binding protein (MCaBP) [29] was isolated by immunoscreening a mouse placenta cDNA library constructed in the expression phage vector, lambda gt 11. The MCaBP cDNA was 0.7 kb in size, with restriction sites for StuI and Bg/II, and its identity to the MCaBP was confirmed by mRNA hybrid selection. RNA blot hybridization revealed a predominant, 3.9-kb transcript of the MCaBP in day-18 mouse placenta. The expression of the MCaBP during development was analyzed with respect to the levels of protein activity, translatable MCaBP mRNA, and total MCaBP transcript.