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Nucleic acid sequence relationships between enterovirus serotypes.

T Hyypiä1, M Maaronen, P Auvinen

  • 1Department of Virology, University of Turku, Finland.

Molecular and Cellular Probes
|June 1, 1987
PubMed
Summary
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Nucleic-acid hybridization effectively detects over 90% of enterovirus serotypes. This method aids in rapid enterovirus detection and subgrouping during virus isolation, showing potential for strain typing.

Area of Science:

  • Virology
  • Molecular Biology
  • Infectious Diseases

Background:

  • Enteroviruses encompass a large group of viruses causing various human diseases.
  • Accurate and rapid identification of enterovirus serotypes is crucial for diagnosis and epidemiology.
  • Existing methods for enterovirus identification can be time-consuming and complex.

Purpose of the Study:

  • To evaluate the utility of nucleic-acid hybridization for detecting and subgrouping diverse enterovirus serotypes.
  • To assess the effectiveness of probes derived from coxsackievirus A21, B3, poliovirus 3, and enterovirus 70.
  • To determine the potential of this method for rapid enterovirus strain typing.

Main Methods:

  • Analysis of 48 enterovirus serotypes using nucleic-acid hybridization.

Related Experiment Videos

  • Utilized probes derived from the 3' end of coxsackievirus A21 (CA21), coxsackievirus B3 (CB3), poliovirus 3 (P3), and enterovirus 70 (E70).
  • Examined hybridization patterns for each probe against different enterovirus types.

    • Main Results:

    • The nucleic-acid hybridization test detected over 90% of the analyzed enterovirus serotypes.
    • The CB3 probe demonstrated broad reactivity, detecting coxsackie B, poliovirus, ECHO viruses, and some coxsackie A and enterovirus 71 strains.
    • The P3 and CA21 probes showed similar patterns, detecting poliovirus and some coxsackievirus A isolates, while the E70 probe was strain-specific.

    Conclusions:

    • Nucleic-acid hybridization is a valuable tool for the rapid detection and subgrouping of enteroviruses.
    • The developed probe collection facilitates efficient identification of a wide range of enterovirus serotypes.
    • Further development of this hybridization test holds promise for precise enterovirus strain typing.