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Related Concept Videos

FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

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Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
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In-situ Hybridization02:31

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In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
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In Situ Hybridization for the Precise Localization of Transcripts in Plants
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In situ hybridization.

T R Moench1

  • 1Department of Medicine, Johns Hopkins School of Medicine, Baltimore, Maryland 21205.

Molecular and Cellular Probes
|September 1, 1987
PubMed
Summary
This summary is machine-generated.

In situ hybridization detects specific nucleic acid sequences within cells and tissues, offering unique localization and high sensitivity for DNA and RNA analysis across diverse scientific fields.

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Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • In situ hybridization (ISH) is a technique for detecting specific nucleic acid sequences within intact cells or tissues.
  • It provides spatial localization of nucleic acids, correlating with cellular structures.
  • Traditional hybridization methods lack this direct anatomical context.

Purpose of the Study:

  • To highlight the capabilities and applications of in situ hybridization.
  • To emphasize its advantages over other hybridization techniques.
  • To showcase its versatility and sensitivity in nucleic acid detection.

Main Methods:

  • Hybridization-mediated detection of specific DNA or RNA sequences.
  • Application to structurally intact cells or tissues.
  • Analysis at macroscopic, light microscopic, or electron microscopic levels.

Main Results:

  • In situ hybridization offers unique localization of nucleic acids within cellular structures.
  • The technique demonstrates high sensitivity, especially for low-abundance targets.
  • Innovations have expanded its versatility for detecting DNA/RNA (sense/anti-sense) and various sample preparations.

Conclusions:

  • In situ hybridization is a powerful tool for analyzing nucleic acid distribution in biological samples.
  • Its ability to combine molecular detection with structural detail makes it invaluable.
  • The technique is broadly applicable across diverse scientific disciplines.