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Engineering interaction between bone marrow derived endothelial cells and electrospun surfaces for artificial
Furqan Ahmed1, Naba K Dutta, Andrew Zannettino
1Ian Wark Research Institute, University of South Australia , Mawson Lakes Campus, South Australia, Australia.
Biomacromolecules
|February 26, 2014
Summary
Electrospun PVDF-HFP nanofiber surfaces, when coated with fibrinogen, significantly enhance endothelial cell interactions, showing promise for artificial vascular graft applications.
Area of Science:
- Biomaterials Engineering
- Tissue Engineering
- Cell Biology
Background:
- Developing functional small diameter artificial vascular grafts (SDAVG) is crucial for cardiovascular disease treatment.
- Polyvinylidene fluoride-co-hexafluoropropylene (PVDF-HFP) is a candidate material, but its surface properties need optimization for endothelialization.
- Understanding cell-surface interactions is key to designing effective biomaterials.
Purpose of the Study:
- To investigate endothelial cell interactions with electrospun (ES) and solvent cast (SC) PVDF-HFP nanofiber surfaces.
- To evaluate the effect of fibrinogen adsorption on endothelialization of these surfaces.
- To assess the potential of engineered PVDF-HFP surfaces for vascular graft applications.
Main Methods:
- Fabrication of PVDF-HFP samples via electrospinning (ES) and solvent casting (SC).
- Adsorption of fibrinogen onto ES and SC surfaces.
- Incubation of human bone marrow-derived endothelial cells (BMEC) with pristine and fibrinogen-adsorbed surfaces.
- Assessment of BMEC attachment, proliferation, and viability using various techniques.
- Analysis of fibrinogen-surface interactions using ELISA, XPS, and FTIR.
Main Results:
- Pristine SC and ES PVDF-HFP surfaces exhibited hydrophobic properties and minimal BMEC growth.
- Fibrinogen adsorption did not significantly improve endothelial cell binding or proliferation on SC surfaces.
- Fibrinogen-adsorbed ES surfaces demonstrated a notable ability to modulate endothelial cell behavior, enhancing attachment and proliferation.
- ES surfaces with adsorbed fibrinogen showed potential for promoting endothelialization.
Conclusions:
- Electrospun PVDF-HFP nanofiber surfaces, particularly when modified with fibrinogen, offer a promising platform for endothelialization.
- Engineered PVDF-HFP surfaces can be optimized to promote favorable endothelial cell interactions for vascular graft applications.
- This study provides insights into cell-biomaterial interactions, crucial for designing next-generation vascular grafts.
