Lamina X of primate spinal cord: distribution of five neuropeptides and serotonin

The distribution of substance P, somatostatin, cholecystokinin, vasoactive intestinal polypeptide, enkephalin and serotonin in axons, terminals and neurons was compared in the area surrounding the central canal (lamina X) at five representative levels of the monkey spinal cord, using peroxidase-antiperoxidase immunocytochemistry. Immunoreactive neurons containing each of the neurochemicals were identified. At the cervical, thoracic and lumbar levels the area lateral to the canal had dense terminal fields immunoreactive for each neurochemical. The dorsal commissural region, the pericanal area, and the ventral commissural area were supplied by some but not all of the substances. In the lower thoracic cord innervation extended into the dorsal midline area and into the ventromedial commissural region. In contrast, in the sacral cord, the dorsal commissural region could be subdivided on the basis of innervation, and the lateral region was densely supplied by only cholecystokinin and serotonin, while the sacral ventral commissure and the pericanal area were supplied by all six neurochemicals. The immunocytochemical mappings were compared with published maps of functional classes of neurons and with the distribution of primary afferents and descending fibers in lamina X. The dense peptidergic and serotonergic innervation in the lateral area and the dorsal commissural area corresponded particularly with the location of projection neurons and primary afferents described in other studies.