Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Kainic acid lesions decrease striatal dopamine receptors and 1,4-dihydropyridine sites.

A Skattebøl1, R E Hruska, M Hawthorn

  • 1Department of Biochemical Pharmacology, School of Pharmacy, State University of New York, Buffalo 14260.

Neuroscience Letters
|June 17, 1988
PubMed
Summary

Intrastriatal kainic acid injections in rats reduced dopamine receptors by 58% and 1,4-dihydropyridine sites by 43%. This suggests 1,4-dihydropyridine-sensitive Ca2+ channels are primarily on postsynaptic elements.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Overview of chemical diversity.

Current protocols in pharmacology·2011
Same author

Addiction.

IDrugs : the investigational drugs journal·2005
Same author

Division of Medicinal Chemistry--integrins: past, present and future.

IDrugs : the investigational drugs journal·2005
Same author

[No one is avoiding the General Practice Research Committee].

Tidsskrift for den Norske laegeforening : tidsskrift for praktisk medicin, ny raekke·2002
Same author

Interaction of azimilide with neurohumoral and channel receptors.

Biochemical pharmacology·2001
Same author

Regulation of L-type calcium channels in pituitary GH(4)C(1) cells by depolarization.

The Journal of biological chemistry·2001

Area of Science:

  • Neuroscience
  • Neuropharmacology
  • Cell Biology

Background:

  • Kainic acid is a neurotoxin that selectively damages neurons, particularly in the striatum.
  • Dopamine receptors and 1,4-dihydropyridine sites are crucial for neurotransmission and calcium signaling.
  • Understanding the cellular localization of these sites is important for neurological research.

Purpose of the Study:

  • To investigate the effects of kainic acid-induced lesions on dopamine receptor and 1,4-dihydropyridine binding in the rat striatum.
  • To determine the impact of these lesions on the density and affinity of these binding sites.
  • To explore the potential postsynaptic localization of 1,4-dihydropyridine-sensitive Ca2+ channels.

Main Methods:

  • Intrastriatal injection of kainic acid in rats.

Related Experiment Videos

  • Radioligand binding assays using [3H]spiperone for dopamine receptors.
  • Radioligand binding assays using [3H]nimodipine for 1,4-dihydropyridine sites.
  • Analysis of binding densities and affinities four weeks post-lesioning.

    • Main Results:

    • Kainic acid lesions significantly decreased dopamine receptor binding density by 58%.
    • A 43% reduction in 1,4-dihydropyridine binding density was observed.
    • No significant changes in the binding affinity for either radioligand were detected.
    • These findings indicate a substantial loss of these specific binding sites following striatal damage.

    Conclusions:

    • Intrastriatal kainic acid lesions lead to a significant downregulation of striatal dopamine receptors and 1,4-dihydropyridine binding sites.
    • The data suggest that 1,4-dihydropyridine-sensitive Ca2+ channels are predominantly located on postsynaptic neuronal elements in the striatum.
    • This study provides insights into the cellular targets affected by excitotoxic injury and the localization of calcium channels.