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Updated: May 2, 2026
Isolation, Culture, and Characterization of Dental Pulp Stem Cells from Human Deciduous and Permanent Teeth
Published on: May 17, 2024
Methylation pattern of the CD14 and TLR2 genes in human dental pulp
Fabiano Pereira Cardoso1, Simone Angélica de Faria Amormino2, Walderez Ornelas Dutra2
1Department of Restorative Dentistry, Faculdade de Odontologia, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
Introduction:
Pattern recognition receptors, such as toll-like receptor 2 (TLR-2) and TLR-4, participate in the activation of immune cells by microorganisms in dental pulp. However, the expression levels of pattern recognition receptors can be modulated by epigenetic factors, especially DNA methylation. In this study, the methylation status of the TLR-2 and CD14 (TLR4 co-receptor) genes in healthy and inflamed human dental pulp was examined.
Methods:
The Methyl-Profiler DNA Methylation qPCR Assay was used to verify the DNA methylation patterns.
Results:
No differences in the methylation patterns were observed between the 2 groups. Most DNA was unmethylated in both groups.
Conclusions:
The hypomethylation of TLR2 and CD14 genes is a usual feature in human dental pulp.
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