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Published on: March 30, 2019
TRIM31 is downregulated in non-small cell lung cancer and serves as a potential tumor suppressor
1Department of Geriatrics, Shengjing Hospital of China Medical University, 36 Sanhao Road, Shenyang, 110004, China.
Abstract:
The present study aims to investigate expression pattern and biological roles of TRIM31 in human non-small cell lung cancer (NSCLC). We examined TRIM31 expression in 116 NSCLC tissues and 20 corresponding normal lung tissues by immumohistochemistry. We found TRIM31 downregulation in 47 out of 116 (40.5 %) cancer samples, which correlated with tumor status (p=0.0132), advanced p-TNM stage (p=0.001), and nodal metastasis (p=0.0382). TRIM31 expression was lower in lung cancer cell lines than normal bronchial cell line HBE. Transfection of TRIM31 plasmid was performed in H157 and H1299 cells. TRIM31 overexpression inhibited cell growth rate and colony formation ability in both cell lines. In addition, expression of cell cycle regulator cyclin D1 and cyclin E were decreased after TRIM31 transfection. In conclusion, TRIM31 might serve as a tumor suppressor in non-small cell lung cancer.
Insights
TRIM31 is often downregulated in non-small cell lung cancer (NSCLC). Restoring TRIM31 expression suppresses tumor growth and cell proliferation, suggesting TRIM31 acts as a tumor suppressor in NSCLC.
Area of Science:
- Oncology
- Molecular Biology
Background:
- Non-small cell lung cancer (NSCLC) is a leading cause of cancer-related mortality.
- Understanding the molecular mechanisms underlying NSCLC progression is crucial for developing effective therapies.
Purpose of the Study:
- To investigate the expression pattern of TRIM31 in NSCLC.
- To elucidate the biological roles of TRIM31 in NSCLC progression.
Main Methods:
- Immunohistochemistry was used to examine TRIM31 expression in 116 NSCLC tissues and 20 normal lung tissues.
- TRIM31 plasmid transfection was performed in NSCLC cell lines (H157 and H1299).
- Cell growth rate, colony formation ability, and cell cycle regulators (cyclin D1, cyclin E) were assessed.
Main Results:
- TRIM31 was downregulated in 40.5% of NSCLC samples.
- TRIM31 downregulation correlated with advanced tumor status, p-TNM stage, and nodal metastasis.
- TRIM31 overexpression inhibited NSCLC cell growth, colony formation, and reduced cyclin D1 and cyclin E expression.
Conclusions:
- TRIM31 exhibits tumor-suppressive functions in NSCLC.
- TRIM31 downregulation is associated with aggressive tumor characteristics in NSCLC.
- TRIM31 may represent a potential therapeutic target for NSCLC.
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