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Caspases

Caspases

Caspase, a family of cysteine proteases, serve as effectors in apoptosis. The ced3 gene in C.elegans was first identified to be involved in apoptosis. This gene encodes the ced-3 caspase that is similar to the interleukin-1-beta converting enzyme or ICE in mammals. In addition to apoptosis, caspases also function in the inflammatory response. Inflammatory caspases are essential in activating pro-inflammatory cytokines that recruit immune cells and block the replication of pathogens inside...

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Updated: May 2, 2026

Evaluation of Caspase Activation to Assess Innate Immune Cell Death

Evaluation of Caspase Activation to Assess Innate Immune Cell Death

Published on: January 20, 2023

Caspase-14 protocols.

Mami Yamamoto-Tanaka¹, Toshihiko Hibino

¹Shiseido Research Center, Tsuzuki-ku, Yokohama, Japan.

Methods in Molecular Biology (Clifton, N.J.)

|February 26, 2014

Summary

This summary is machine-generated.

Caspase-14, crucial for skin barrier function, is activated during corneocyte formation. This study details methods to measure and visualize active caspase-14 in skin cells.

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Last Updated: May 2, 2026

Evaluation of Caspase Activation to Assess Innate Immune Cell Death

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Published on: January 20, 2023

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Measuring Caspase Activity Using a Fluorometric Assay or Flow Cytometry

Published on: March 24, 2023

Area of Science:

Biochemistry
Dermatology
Molecular Biology

Background:

Caspase-14 is primarily expressed in the epidermis and involved in keratinocyte differentiation.
Unlike other caspases, caspase-14 is not typically associated with apoptosis or inflammation.
Its activation is linked to the formation of corneocytes, a key step in skin barrier development.

Purpose of the Study:

To establish and present methodologies for assessing caspase-14 activity and presence.
To provide tools for further research into caspase-14's role in skin biology.
To detail the purification, activation, and quantification of caspase-14.

Main Methods:

Purification of active caspase-14 from human corneocyte extracts.
Development of techniques to measure caspase-14 activity using synthetic substrates.
Preparation of constitutively active caspase-14 and specific antibodies.
Quantification of total and active caspase-14 via ELISA.
Methods for visualizing caspase-14 activation using immunohistochemistry.

Main Results:

Successful purification of active caspase-14 from human corneocytes.
Established methods for measuring caspase-14 activity and quantifying its levels.
Developed tools (antibody, active enzyme) for further caspase-14 research.
Demonstrated visualization of caspase-14 activation in situ.

Conclusions:

The study provides a comprehensive toolkit for investigating caspase-14.
These methods facilitate deeper understanding of caspase-14's function in keratinocyte terminal differentiation.
Further research into caspase-14 is enabled by the described techniques.