Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Caspases01:24

Caspases

8.7K
Caspase, a family of cysteine proteases, serve as effectors in apoptosis. The ced3 gene in C.elegans was first identified to be involved in apoptosis. This gene encodes the ced-3 caspase that is similar to the interleukin-1-beta converting enzyme or ICE in mammals. In addition to apoptosis, caspases also function in the inflammatory response. Inflammatory caspases are essential in activating pro-inflammatory cytokines that recruit immune cells and block the replication of pathogens inside...
8.7K
The Intrinsic Apoptotic Pathway01:31

The Intrinsic Apoptotic Pathway

6.2K
Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
6.2K
The Extrinsic Apoptotic Pathway01:17

The Extrinsic Apoptotic Pathway

6.2K
The extrinsic apoptotic pathway is initiated when extracellular death-inducing signals, such as specific cytokines, activate the death receptors expressed on the cell surface. The immune cells involved in this pathway are natural killer cells (NK cells) and cytotoxic T-lymphocytes. NK cells are critical in innate immune response, while cytotoxic T-lymphocytes are associated with adaptive immune response. These cells recognize specific receptors expressed on the altered cells and activate...
6.2K
Apoptosis01:30

Apoptosis

12.0K
Apoptosis is a combination of two Greek words, 'apo' and 'ptosis,' meaning separation and falling off, respectively. Hippocrates used this word to describe gangrene, which was caused due to bandaging of fractured bones. Apoptosis was distinguished from necrosis in 1970 when John Kerr reported observations of morphological changes occurring during apoptosis. During one experiment, he observed that the disruption of blood supply to the liver tissue resulted in a size...
12.0K
MAPK Signaling Cascades01:07

MAPK Signaling Cascades

7.3K
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
7.3K
Anaphase Promoting Complex00:50

Anaphase Promoting Complex

2.5K
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
2.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Shedding light on plant proteolysis: genetically encoded fluorescent sensors as tools for profiling protease activities.

The Plant cell·2026
Same author

A dual phospholipase system instructs membrane hydrolysis during the final stages of plant autophagy.

Nature communications·2026
Same author

Integrating natural variation through GWAS - genetics of drought and flood tolerance in grass pea reveal independent yet interconnected mechanisms.

BMC plant biology·2026
Same author

Investigating the origin of spontaneous cytosolic calcium oscillations in stomatal guard cells: a dialogue between apoplastic and endoplasmic reticulum stores.

The New phytologist·2025
Same author

FUSTer than stress granules: a prion-like domain warns plants of heat.

Cell research·2025
Same author

The burning glass effect of water droplets triggers a high light-induced calcium response in the chloroplast stroma.

Current biology : CB·2025

Related Experiment Video

Updated: May 2, 2026

Measuring Caspase Activity Using a Fluorometric Assay or Flow Cytometry
05:29

Measuring Caspase Activity Using a Fluorometric Assay or Flow Cytometry

Published on: March 24, 2023

7.7K

Plant metacaspase activation and activity.

Elena A Minina1, Simon Stael, Frank Van Breusegem

  • 1Department of Plant Biology, Uppsala BioCenter, Swedish University of Agricultural Sciences and Linnean Center for Plant Biology, Uppsala, Sweden.

Methods in Molecular Biology (Clifton, N.J.)
|February 26, 2014
PubMed
Summary

This study presents new methods to detect metacaspase activation and measure their proteolytic activity in plants. These techniques are crucial for understanding plant cell death regulation and can be adapted for various metacaspase types.

More Related Videos

Evaluation of Caspase Activation to Assess Innate Immune Cell Death
10:23

Evaluation of Caspase Activation to Assess Innate Immune Cell Death

Published on: January 20, 2023

3.6K
Lighting Up the Pathways to Caspase Activation Using Bimolecular Fluorescence Complementation
08:47

Lighting Up the Pathways to Caspase Activation Using Bimolecular Fluorescence Complementation

Published on: March 5, 2018

8.5K

Related Experiment Videos

Last Updated: May 2, 2026

Measuring Caspase Activity Using a Fluorometric Assay or Flow Cytometry
05:29

Measuring Caspase Activity Using a Fluorometric Assay or Flow Cytometry

Published on: March 24, 2023

7.7K
Evaluation of Caspase Activation to Assess Innate Immune Cell Death
10:23

Evaluation of Caspase Activation to Assess Innate Immune Cell Death

Published on: January 20, 2023

3.6K
Lighting Up the Pathways to Caspase Activation Using Bimolecular Fluorescence Complementation
08:47

Lighting Up the Pathways to Caspase Activation Using Bimolecular Fluorescence Complementation

Published on: March 5, 2018

8.5K

Area of Science:

  • Plant Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Metacaspases are key regulators of plant cell death.
  • Understanding their biochemical activity is vital for plant biology research.
  • Robust methods for detecting metacaspase activation and activity are needed.

Purpose of the Study:

  • To develop and describe robust methods for detecting metacaspase activation.
  • To enable quantitative analysis of metacaspase proteolytic activity.
  • To provide tools for studying plant cell death regulation.

Main Methods:

  • Purification of recombinant metacaspases.
  • Measurement of enzymatic activity in vitro and in cell lysates.
  • In vivo detection of metacaspase activation.
  • In vitro substrate cleavage assay using cell-free produced proteins.

Main Results:

  • Established methods for recombinant and endogenous metacaspase activity measurement.
  • Developed assays for in vivo metacaspase activation detection.
  • Presented a cell-free based substrate cleavage assay.
  • Methods validated for Arabidopsis and Norway spruce metacaspases.

Conclusions:

  • The described methods offer a comprehensive toolkit for studying plant metacaspases.
  • These techniques can be adapted for diverse metacaspase types (I and II) across species.
  • Facilitates deeper understanding of metacaspase function in plant cell death.