Immunoblotting analyses of changes in protein phosphorylations during oocyte maturation in marine nemertean worms

Immunoblotting analyses combined with phospho-specific antibodies can provide a powerful means for assessing protein activity states in various cellular extracts. This chapter describes a traditional, film-based immunoblotting method for monitoring the phosphorylation status of proteins in marine nemertean oocytes undergoing maturation. Similarly, with minor modifications, the protocol could potentially be applied to a wider variety of cellular processes and extract types that might be analyzed in other investigations of marine invertebrate development.