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Related Experiment Video

Updated: May 2, 2026

Investigating Intestinal Barrier Breakdown in Living Organoids
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Deoxynivalenol: a trigger for intestinal integrity breakdown.

Peyman Akbari1, Saskia Braber2, Hendrik Gremmels3

  • 1Division of Veterinary Pharmacy, Pharmacology, and Toxicology, and Division of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Faculty of Science, Utrecht University, The Netherlands;

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
|February 27, 2014
PubMed
Summary
This summary is machine-generated.

The fungal toxin deoxynivalenol (DON) disintegrates the intestinal epithelial barrier by disrupting tight junctions. This finding suggests DON may trigger inflammatory bowel and celiac diseases.

Keywords:
Caco-2 cellsTEERtight junction proteins

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Area of Science:

  • Gastroenterology
  • Toxicology
  • Cell Biology

Background:

  • Colonic epithelial barrier disintegration is crucial in inflammatory bowel and celiac diseases.
  • The exact causes of these conditions remain unknown.
  • Fungal metabolite deoxynivalenol (DON) is present in grain-based diets.

Purpose of the Study:

  • To investigate if deoxynivalenol (DON) triggers intestinal barrier impairment.
  • To explore DON's role in initiating inflammatory bowel and celiac diseases.

Main Methods:

  • Horizontal impedance measurements on Caco-2 cell monolayers.
  • Assessing Caco-2 cell permeability using lucifer yellow and FITC-dextran.
  • In vivo studies using B6C3F1 mice orally challenged with DON.
  • Analyzing claudin and ZO-1 protein and mRNA levels.

Main Results:

  • DON rapidly disintegrated Caco-2 cell monolayers at low concentrations (1.39 μM).
  • DON exposure decreased transepithelial resistance and increased intestinal permeability in vitro and in vivo.
  • DON reduced claudin protein levels and altered their cellular localization.
  • Compensatory upregulation of claudin and ZO-1 mRNA was observed.
  • DON-treated mice exhibited villus architecture alterations resembling inflammatory bowel disease.

Conclusions:

  • Deoxynivalenol (DON) directly disrupts the intestinal epithelial barrier by affecting tight junctions.
  • DON may act as a trigger for inflammatory bowel and celiac diseases.
  • Further research into DON's role in gastrointestinal inflammation is warranted.