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A requirement for the CD5 antigen in T cell activation
M J McAteer1, A C Lagarde, H M Georgiou
1Barbara Davis Center for Childhood Diabetes, Department of Microbiology and Immunology, University of Colorado Health Sciences Center, Denver 80262.
European Journal of Immunology
|July 1, 1988
Summary
Monoclonal antibody treatment depleted CD5+ cells in rats, creating CD5- T cells that lost proliferative capacity. Re-expressing CD5 restored T cell proliferation, indicating its crucial role in resting T cell activation.
Area of Science:
- Immunology
- Cell Biology
Background:
- The CD5 antigen is a cell surface glycoprotein expressed on T lymphocytes.
- The precise function of CD5 in T cell activation and function remains incompletely understood.
Purpose of the Study:
- To investigate the role of the CD5 antigen in T cell proliferation and function.
- To determine if CD5 expression is essential for T cell responses.
Main Methods:
- Treatment of adult rats with a CD5-specific monoclonal antibody to deplete CD5+ T cells.
- Assessment of T cell function, including proliferation, cytotoxicity, and graft-vs.-host disease induction.
- Evaluation of CD5 re-expression and its effect on T cell responses in vitro.
Main Results:
- Monoclonal antibody treatment significantly reduced CD5+ T cells, generating a CD5- T cell population.
- CD5- T cells exhibited impaired proliferation to alloantigen and mitogens, and failed to induce graft-vs.-host disease.
- Re-expression of CD5 on CD5- T cells restored proliferative responses.
- Interleukin 2 production was observed in CD5- T cells upon mitogenic stimulation, but exogenous IL-2 did not rescue proliferative defects.
Conclusions:
- The CD5 antigen is essential for the proliferative capacity of resting T cells.
- CD5 plays a critical role in T cell activation pathways beyond its potential involvement in interleukin 2 signaling.