Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

16.8K
Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
16.8K
Labeling DNA Probes03:31

Labeling DNA Probes

7.7K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
7.7K
Southern Blot02:57

Southern Blot

14.9K
Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...
14.9K
FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

17.3K
Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
17.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Single-molecule force measurements show that r-proteins assist 23S rRNA co-transcriptional folding.

Biophysical journal·2026
Same author

DNA Hybridization Measured with Graphene Transistor Arrays.

Advanced healthcare materials·2020
Same author

RNA Folding and Unfolding Under Force: Single-Molecule Experiments and Their Analysis.

Methods in molecular biology (Clifton, N.J.)·2020
Same author

Overstretching Double-Stranded RNA, Double-Stranded DNA, and RNA-DNA Duplexes.

Biophysical journal·2019
Same author

Force measurements show that uL4 and uL24 mechanically stabilize a fragment of 23S rRNA essential for ribosome assembly.

RNA (New York, N.Y.)·2019
Same author

Single-Molecule FRET Assay to Observe the Activity of Proteins Involved in RNA/RNA Annealing.

Methods in molecular biology (Clifton, N.J.)·2018

Related Experiment Video

Updated: May 2, 2026

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
14:53

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

16.4K

Electronic hybridization detection in microarray format and DNA genotyping.

Antoine Blin1, Ismaïl Cissé1, Ulrich Bockelmann1

  • 1Laboratoire Nanobiophysique ESPCI ParisTech, CNRS UMR Gulliver 7083 10 rue Vauquelin, 75005 Paris, France.

Scientific Reports
|February 27, 2014
PubMed
Summary

We developed a novel semiconductor device to replace fluorescence microarrays for genetic mutation detection. This new method accurately identifies the 35delG mutation linked to deafness using electrolyte-gated field-effect transistors.

More Related Videos

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning
09:27

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning

Published on: March 15, 2011

40.5K
Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization
16:37

Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization

Published on: August 5, 2008

12.5K

Related Experiment Videos

Last Updated: May 2, 2026

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
14:53

A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis

Published on: September 10, 2014

16.4K
DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning
09:27

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning

Published on: March 15, 2011

40.5K
Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization
16:37

Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization

Published on: August 5, 2008

12.5K

Area of Science:

  • Biotechnology
  • Semiconductor device technology
  • Molecular diagnostics

Background:

  • Fluorescence microarrays are standard tools for genetic analysis but can be limited by background fluorescence and require specialized equipment.
  • The 35delG mutation is a common cause of non-syndromic deafness, necessitating efficient and accurate detection methods.
  • Developing alternative detection platforms is crucial for advancing genetic diagnostics.

Purpose of the Study:

  • To introduce a novel approach for genetic mutation detection using electrolyte-gated field-effect transistors (EGFETs) as a substitute for fluorescence microarrays.
  • To demonstrate the efficacy of this EGFET-based system for detecting the specific 35delG mutation associated with deafness.
  • To validate a new polymerase chain reaction (PCR) technique for generating unique DNA barcodes for detection.

Main Methods:

  • Fabrication of a semiconductor surface comprising an arrangement of EGFETs.
  • Implementation of a blocking strategy to minimize non-specific interactions on the transistor surface.
  • Utilizing a newly developed polymerase chain reaction (Tas-PCR) with polymerase blocking to generate barcode sequences.
  • Hybridization of barcode sequences to surface-attached probes and direct detection of threshold voltage shifts in EGFETs.

Main Results:

  • The EGFET-based surface successfully replaced traditional fluorescence microarrays.
  • Threshold voltage shifts in EGFETs correlated with the presence of specific probe molecules, enabling sequence discrimination.
  • The system demonstrated successful detection of the 35delG mutation using Tas-PCR-generated barcodes.

Conclusions:

  • Electrolyte-gated field-effect transistors offer a viable alternative to fluorescence microarrays for genetic analysis.
  • The developed method, incorporating Tas-PCR and EGFET detection, provides a sensitive and direct approach for mutation identification.
  • This technology holds promise for improved molecular diagnostics, particularly for genetic disorders like deafness.