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Novel Sequence Discovery by Subtractive Genomics
Published on: January 25, 2019
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Next-generation sequencing strategies for characterizing the turkey genome
Rami A Dalloul1, Aleksey V Zimin, Robert E Settlage
1Avian Immunobiology Laboratory, Department of Animal and Poultry Sciences, Virginia Tech, Blacksburg 24061.
Poultry Science
|February 27, 2014
Summary
The turkey genome sequencing project has significantly improved its assembly and annotation since 2010. Current efforts cover over 95% of the genome, aiding poultry trait research.
Area of Science:
- Genomics
- Comparative Genomics
- Poultry Science
Background:
- The turkey genome sequencing project began in 2008 using next-generation sequencing (NGS).
- Initial assembly (Build 2.01) covered 89% of the genome with 17X coverage.
Purpose of the Study:
- To improve the turkey genome assembly, gene annotation, and genomic analyses.
- To create a valuable resource for studying genetic variations in poultry.
Main Methods:
- Utilized multiple NGS platforms (Roche/454, Illumina GAII, Hi-Seq).
- Employed bacterial artificial chromosome (BAC) maps and advanced assembly tools.
- Incorporated shotgun, paired-end, and targeted sequencing strategies.
- Generated transcriptome sequencing data from various tissues and developmental stages.
Main Results:
- Achieved improved genome builds (3.0, 4.0, 5.0) with increased coverage (up to 30X) and sequence totaling 1,010 Mb.
- Current assembly covers over 95% of the turkey genome.
- Enhanced gene annotation is expected with the latest data.
Conclusions:
- The refined turkey genome assembly provides a significantly improved resource.
- This resource will facilitate research into genetic variations influencing economically important poultry traits.
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