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A conditional high-copy-number plasmid derivative of pBR322
I Ivanov1, L Yavashev, L Gigova
1Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia.
Microbiologica
|April 1, 1988
Abstract:
A spontaneous high-copy-number plasmid derivative of plasmid pBR322 was isolated. This plasmid bears two point mutations adjacent to the unpaired region in the stem of loop II of RNA I and expresses its high-copy-number phenotype only when the harboring cells grow on solid support (L-agar).