Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cadherins in Tissue Organization01:19

Cadherins in Tissue Organization

3.5K
The cadherins are a superfamily of cell adhesion molecules comprising over 180 variants, with specific tissues expressing a particular combination of cadherin types. Cadherins generally exhibit homophilic binding; i.e., cadherins on one cell bind to cadherins of the same or closely related type on another cell. Thus, cells of the same type have a specific affinity to bind to each other and sort themselves into clusters to form tissues.
Cell Sorting During Development
Cell sorting plays an...
3.5K
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

1.8K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
1.8K
Adaptive Mechanisms in Cancer Cells02:53

Adaptive Mechanisms in Cancer Cells

5.7K
Cancer cells accumulate genetic changes at an abnormally rapid rate due to the defects in the DNA repair mechanisms. From an evolutionary perspective, such genetic instability is advantageous for cancer development. Mutant cell lines accumulate a series of beneficial mutations that contribute to their progression into cancer.
Some of the advantages that cancer cells have on normal cells include - enhanced ability to divide without terminally differentiating, induce new blood vessel formation,...
5.7K
Selectins01:25

Selectins

3.6K
Cell adhesion is  an essential aspect of multicellularity. While stable cell interactions usually occur between cells of the same type, transient cell interactions occur between cells of different tissue types, such as between neutrophils and endothelial cells. Selectins are one class of cell adhesion molecules (CAMs) that bind carbohydrate ligands to form transient cell adhesion. They are rod-like proteins with a long extracellular part of variable length ending with the lectin domain,...
3.6K
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal01:22

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

1.8K
Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
1.8K
Cancer Cell Migration through Invadopodia01:35

Cancer Cell Migration through Invadopodia

2.5K
Invadosome is a broad category of cell surface structures with proteolytic activity that  degrades the extracellular matrix (ECM). Invadosomes are present in normal cell types, including macrophages, endothelial cells, and neurons, as well as tumor cells. Although the macrophage podosomes and tumor cell invadopodia are classified as invadosomes, they have different structures, molecular pathways, and functions. Podosomes are short structures that last for a few minutes. However,...
2.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Technological Singularity in Biomedical Science and Engineering.

Cellular and molecular bioengineering·2026
Same author

A <i>Bacillus</i>-based direct-fed microbial mixture remodels the gut microbiome to augment the respiratory health of <i>Salmonella</i>-infected pigs.

Applied and environmental microbiology·2026
Same author

Systems engineering of engineered live biotherapeutics: A discovery-to-translation framework for streamlining microbiome therapeutic development.

Journal of controlled release : official journal of the Controlled Release Society·2026
Same author

Lumpectomy Margins and Local Recurrence in DCIS: Results From the NRG Oncology/NSABP B-35 Randomized Clinical Trial.

JAMA surgery·2026
Same author

Nanoengineered All-Cellulose Bilayer Barrier Papers for High-Performance and Recyclable Food Packaging.

Chem & bio engineering·2026
Same author

Carbon monoxide-releasing molecule-3 eradicates mature <i>Enterococcus faecalis</i> biofilms and inhibits recolonization.

Journal of biomaterials applications·2026

Related Experiment Video

Updated: May 2, 2026

Characterization of Cell Membrane Extensions and Studying Their Roles in Cancer Cell Adhesion Dynamics
08:11

Characterization of Cell Membrane Extensions and Studying Their Roles in Cancer Cell Adhesion Dynamics

Published on: March 26, 2018

8.0K

Dynamic Switch Between Two Adhesion Phenotypes in Colorectal Cancer Cells.

Yue Geng1, Siddarth Chandrasekaran1, Sivaprakash Agastin1

  • 1Department of Biomedical Engineering, Cornell University, Ithaca, NY 14853 USA.

Cellular and Molecular Bioengineering
|February 28, 2014
PubMed
Summary

Colon cancer cells (COLO 205) exhibit tumor heterogeneity, with distinct adherent and suspended subpopulations. Manipulating epithelial-mesenchymal transition markers altered their ratio, impacting cell behavior and interactions.

Keywords:
Cell adhesionE-cadherinE-selectinMetastasisβ-Catenin

More Related Videos

A Genetically Engineered Mouse Model of Sporadic Colorectal Cancer
06:01

A Genetically Engineered Mouse Model of Sporadic Colorectal Cancer

Published on: July 6, 2017

9.1K
A Three-dimensional Model of Spheroids to Study Colon Cancer Stem Cells
06:38

A Three-dimensional Model of Spheroids to Study Colon Cancer Stem Cells

Published on: January 22, 2021

6.0K

Related Experiment Videos

Last Updated: May 2, 2026

Characterization of Cell Membrane Extensions and Studying Their Roles in Cancer Cell Adhesion Dynamics
08:11

Characterization of Cell Membrane Extensions and Studying Their Roles in Cancer Cell Adhesion Dynamics

Published on: March 26, 2018

8.0K
A Genetically Engineered Mouse Model of Sporadic Colorectal Cancer
06:01

A Genetically Engineered Mouse Model of Sporadic Colorectal Cancer

Published on: July 6, 2017

9.1K
A Three-dimensional Model of Spheroids to Study Colon Cancer Stem Cells
06:38

A Three-dimensional Model of Spheroids to Study Colon Cancer Stem Cells

Published on: January 22, 2021

6.0K

Area of Science:

  • Oncology
  • Cell Biology
  • Biophysics

Background:

  • Hematogenous metastasis involves cancer cell interactions with endothelial cells.
  • Tumor heterogeneity is crucial for understanding cancer progression and metastasis.
  • The COLO 205 colon cancer cell line exhibits both adherent and suspended growth, modeling tumor heterogeneity.

Purpose of the Study:

  • To investigate the dynamic interplay between adherent and suspended subpopulations of COLO 205 colon cancer cells.
  • To characterize the molecular differences and interaction capabilities of these subpopulations.
  • To explore the role of epithelial-mesenchymal transition (EMT) in regulating cell state dynamics.

Main Methods:

  • Culturing COLO 205 cells to observe adherent/suspended ratios and equilibrium.
  • Analyzing E-selectin binding glycans and cell-E-selectin interactions under flow.
  • Modulating epithelial-mesenchymal transition (EMT) markers (β-catenin, E-cadherin) using siRNA and antibody-coated microbeads.
  • Supplementing media with human plasma to assess its effect on cell ratios.

Main Results:

  • COLO 205 cells maintain a stable 7:3 adherent to suspended ratio in culture.
  • Adherent cells show reduced E-selectin binding glycans and weaker interactions with E-selectin compared to suspended cells.
  • EMT marker manipulation shifted the ratio to 9:1 (adherent-favored), while human plasma shifted it to 1:9 (suspended-favored).

Conclusions:

  • The COLO 205 cell line serves as a valuable in vitro model for studying dynamic cell population switching and tumor heterogeneity.
  • Differential phenotypes and interactions of subpopulations are linked to EMT processes.
  • Environmental factors like human plasma can significantly influence cell state dynamics.