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Treatment of Platelet Products with Riboflavin and UV Light: Effectiveness Against High Titer Bacterial Contamination

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Methylene blue based device for pathogen reduction in human plasma.

A Elikaei¹, S M Hosseini², Z Sharifi¹

¹Virology Laboratory, Research Centre of Iranian Blood Transfusion Organization (IBTO), Tehran, Iran.

Iranian Journal of Pediatric Hematology and Oncology

|February 28, 2014

Summary

This study developed a device using Methylene Blue (MB) and red LED light to inactivate viruses in plasma. The technology effectively reduced model viruses, meeting World Health Organization (WHO) safety recommendations for pathogen reduction.

Keywords:

Blood-borne Pathogens Methylene Blue Plasma

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Area of Science:

Biomedical Engineering
Infectious Disease Control
Blood Safety

Background:

Plasma transfusion safety is a global concern due to residual virus transmission risks.
Pathogen Reduction Technologies (PRT) are crucial for mitigating these risks, with Methylene Blue (MB) based methods being widely adopted.
The World Health Organization (WHO) recommends PRT to enhance the safety of blood products.

Purpose of the Study:

To develop and evaluate a novel device for virus inactivation in plasma using Methylene Blue (MB) and light.
To assess the efficacy of the developed device against model viruses under varying conditions.

Main Methods:

An interventional study utilizing red Light Emitting Diodes (LEDs) (627 nm) to illuminate plasma samples in the presence of Methylene Blue (MB).
Two model viruses, Herpes Simplex Virus (HSV) and Vesicular Stomatitis Virus (VSV), were used to quantify viral log reduction via TCID50.
Experiments were conducted using two MB concentrations (10 µM and 1 µM) and five different illumination times.

Main Results:

At 10 µM MB, maximum log reductions of 6.00±0.2 for HSV (60 min) and 5.50±0.3 for VSV (75 min) were achieved.
At 1 µM MB, maximum log reductions of 5.20±0.3 for HSV (60 min) and 4.90±0.2 for VSV (75 min) were observed.
The results demonstrated significant viral inactivation across tested conditions.

Conclusions:

The developed device effectively inactivates model viruses in plasma using Methylene Blue and LED illumination.
The observed virus inactivation levels are comparable to existing PRT methods, meeting WHO safety standards.
This technology presents a viable option for enhancing plasma product safety and preventing transfusion-transmitted infections.