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Endogenous digitalislike circulating substances in spontaneously hypertensive rats
I Wauquier1, M G Pernollet, M L Grichois
1Department of Pharmacology, INSERM U7, Paris, France.
Hypertension (Dallas, Tex. : 1979)
|August 1, 1988
Summary
Elevated levels of circulating digitalislike compounds were found in spontaneously hypertensive rats (SHR) as early as 3-4 weeks old. These compounds may play a role in hypertension development.
Area of Science:
- Cardiovascular Physiology
- Endocrinology
- Hypertension Research
Background:
- Digitalis-like compounds are implicated in Na+-dependent hypertension.
- Their role in essential hypertension requires further investigation.
Purpose of the Study:
- To investigate circulating Na+-K+ pump inhibitors in spontaneously hypertensive rats (SHR).
- To compare inhibitor levels in SHR with normotensive Wistar-Kyoto (WKY) and Wistar rats.
Main Methods:
- Assessed plasma's inhibition of Na+ efflux from erythrocytes.
- Measured cross-reactivity with digoxin antibodies.
- Evaluated plasma extract inhibition of kidney Na+,K+-adenosine triphosphatase (ATPase) activity.
Main Results:
- SHR plasma showed a 27% greater inhibition of Na+ efflux compared to WKY/Wistar plasma.
- SHR plasma exhibited double the digoxin antibody cross-reactivity.
- Plasma extracts from SHR significantly inhibited Na+,K+-ATPase activity versus WKY.
Conclusions:
- Circulating digitalislike compounds are elevated in SHR from an early age (3-4 weeks).
- The precise role of these compounds in blood pressure regulation needs further elucidation.