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Related Concept Videos

Biosynthesis of Polysaccharides01:26

Biosynthesis of Polysaccharides

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Polysaccharides such as glycogen and starch are synthesized from nucleoside diphosphate sugars, primarily uridine diphosphate glucose (UDPG) and adenosine diphosphate glucose (ADPG). These activated glucose donors act as key intermediates in carbohydrate metabolism and biosynthesis. UDPG primarily involves glycogen synthesis in animals and many bacteria, while ADPG plays a fundamental role in starch synthesis in plants and certain bacteria.UDPG is formed when glucose-1-phosphate reacts with...
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Upstream Processing01:27

Upstream Processing

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Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...
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Related Experiment Video

Updated: May 2, 2026

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis
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Quantitative high throughput analytics to support polysaccharide production process development.

Aaron Noyes1, Ranga Godavarti2, Nigel Titchener-Hooker3

  • 1The Advanced Centre for Biochemical Engineering, Department of Biochemical Engineering, University College London, Torrington Place, London WC1E 7JE, UK; Pfizer, 1 Burtt Road, Andover, MA 01810, USA.

Vaccine
|March 1, 2014
PubMed
Summary
This summary is machine-generated.

Developing rapid analytical tools for polysaccharide vaccines accelerates high throughput process development (HTPD). New microplate assays measure polysaccharide titre and impurities quickly, reducing process time by 30-fold.

Keywords:
Capsular polysaccharidesCarbohydratesEndotoxin assaysHigh throughput process developmentPhenol sulphuric acid assaySugar quantification

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Area of Science:

  • Biopharmaceutical Manufacturing
  • Analytical Chemistry
  • Vaccine Development

Background:

  • Current analytical methods for polysaccharide vaccines are slow and not suitable for high throughput process development (HTPD).
  • This limits the optimization of manufacturing processes and the development of new vaccine candidates.
  • Faster, automated analytics are crucial for improving vaccine quality and production efficiency.

Purpose of the Study:

  • To develop and qualify rapid, high-throughput analytical assays for polysaccharide vaccine process development.
  • To enable faster screening of polysaccharide feedstreams and impurity profiling.
  • To support the implementation of Quality by Design (QbD) and HTPD in vaccine manufacturing.

Main Methods:

  • Developed rapid microplate assays for polysaccharide titre measurement without heating.
  • Simplified a commercial endotoxin assay for room-temperature, single-measurement use.
  • Qualified assays for protein, nucleic acids, polysaccharide titre, and endotoxins for high-throughput screening.

Main Results:

  • A suite of assays can analyze 96 samples for product concentration and impurity profile in under one day.
  • Assays demonstrated accuracy, precision, robustness, and minimal interference.
  • Reduced overall process time by up to 30-fold and significantly decreased sample volume requirements.

Conclusions:

  • The developed analytical platform provides a foundation for HTPD of polysaccharide vaccines.
  • These advancements facilitate the use of QbD principles in vaccine process development.
  • Faster analytics will accelerate the optimization and creation of new polysaccharide-based vaccines.