Ionic basis of the differential neuronal activity of guinea-pig septal nucleus studied in vitro

1. The electrical properties and ionic conductances of septal neurones were studied by intracellular recording in an in vitro slice preparation. Within the total number of cells recorded (n = 150) we identified three electrophysiological cell types, each one of them located in a separate septal region. Dorsolateral septal neurones comprised 60% of the cells, intermediate septal neurons 10%, and medical septal neurones 30%. 2. Passive electrical constants of dorsolateral, intermediate and medial septal neurones were, respectively:resting potential (-60.2 +/- 4.8, -59.8 +/- 3.3 and -56 +/- 4.3 mV); input resistance (82.5 +/- 17, 63 +/- 16 and 83 +/- 18 M omega) and membrane time constant (18.5 +/- 7.3, 14.2 +/- 6.8 and 10.7 +/- 3.4 ms). 3. Direct activation of dorsolateral septal neurones by current injection below 0.2 nA triggered repetitive firing of fast action potentials. Larger current pulses elicited a characteristic response consisting of an initial fast action potential followed by a train of slow spikes. An after-hyperpolarization followed termination of the pulse and the characteristic response. 4. In dorsolateral septal neurons tetrodotoxin (TTX) abolished the fast action potentials. The slow spikes and the after-hyperpolarization disappeared in presence of Co2+ or after brief removal of external Ca2+. This suggests that the characteristic response is mediated by Ca2+ and the after-hyperpolarization by a Ca2+-dependent K+ conductance. 5. The firing pattern of intermediate septal neurones activated from the resting potential spontaneously measured in the cells was similar to that of dorsolateral septal neurones; but direct activation from a hyperpolarized membrane potential evoked in intermediate septal cells a bursting response due to the generation of a low-threshold spike. The low-threshold spike was TTX-resistant but abolished by Co2+ and reached a maximal amplitude after hyperpolarization to -75 mV lasting for 100-150 ms. These results suggest the existence in intermediate septal neurons of a low-threshold Ca2+ conductance inactivated at the resting potential and deinactivated by hyperpolarization. 6. Depolarization of medial septal neurons by current pulses of amplitude greater than 0.2-0.3 nA elicited a typical burst of two to six action potentials. The bursts lasted for 20-50 ms and were followed by a marked after-hyperpolarization.(ABSTRACT TRUNCATED AT 400 WORDS)