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Glucose metabolism and hexosamine pathway regulate oncogene-induced senescence

D Gitenay1, C Wiel1, H Lallet-Daher1

  • 11] Inserm U1052, Centre de Recherche en Cancérologie de Lyon, Lyon, France [2] CNRS UMR5286, Lyon, France [3] Centre Léon Bérard, Lyon, France [4] Université de Lyon, Lyon, France.

Cell Death & Disease
|March 1, 2014
PubMed

Insights

Oncogenic stress-induced senescence (OIS) involves altered glucose metabolism. Decreased glucose uptake and metabolism promote OIS, while enhancing it helps cells escape senescence, revealing a key role for glucose metabolism in cancer prevention.

Area of Science:

  • Cell Biology
  • Metabolic Regulation
  • Cancer Research

Background:

  • Oncogenic stress-induced senescence (OIS) is a tumor suppression mechanism.
  • Metabolic adaptations are crucial for oncogene-driven proliferation.
  • The role of glucose metabolism in OIS remains largely unexplored, especially in non-excessive glucose conditions.

Purpose of the Study:

  • To investigate the alterations and functional significance of glucose metabolism during OIS in human epithelial cells.
  • To determine how modulating glucose metabolism impacts OIS progression and escape.

Main Methods:

  • Cultivation of human epithelial cells under conditions without glucose excess.
  • Analysis of glucose uptake and metabolism during OIS.
  • Genetic manipulation of key glucose metabolism enzymes (hexokinase 2, glucose-6-phosphatase).
  • Pharmacological inhibition of hexokinase 2.
  • Assessment of OIS and senescence escape following metabolic alterations.

Main Results:

  • A significant slowdown in glucose uptake and metabolism was observed during OIS.
  • Overexpression of hexokinase 2 (HK2) promoted escape from OIS.
  • Inhibition of HK2 or use of non-metabolizable glucose induced premature senescence.
  • The hexosamine pathway emerged as a critical regulator of OIS.

Conclusions:

  • Decreased glucose metabolism is a characteristic feature of OIS.
  • Modulating glucose metabolism, particularly the hexosamine pathway, influences the OIS outcome.
  • Glucose metabolism plays a functional role in the OIS process, impacting tumor suppression.

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