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Updated: May 2, 2026
DNA Vector-based RNA Interference to Study Gene Function in Cancer
Published on: June 4, 2012
Silencing of FOXM1 transcription factor expression by adenovirus-mediated RNA interference inhibits human
1State Key Laboratory of Chemo/Biosensing and Chemometrics, Department of Biomedical Engineering, College of Biology, Collaborative Innovation Center for Chemistry and Molecular Medicine, Hunan University, Changsha, China.
Abstract:
The Forkhead Box M1 (FOXM1) transcription factor has been considered as a potential target for the prevention and/or therapeutic intervention in human carcinomas because of its roles in tumorigenesis and tumor progression through regulating the expression of genes relevant to cell proliferation and transformation. In this study, FOXM1 was found to express strongly in both clinical tissue specimens and human hepatocellular carcinoma (HCC) cell lines such as Huh-6, Huh-7 and HepG2. The knockdown of FOXM1 expression through an adenovirus vector (named AdFOXM1shRNA), which expresses a short hairpin RNA to downregulate FOXM1 expression specifically, diminished the proliferation of Huh-7 and HepG2 cells and anchorage-independent growth of Huh-7 cells. Furthermore, we assessed the efficacy of AdFOXM1shRNA for tumor gene therapy with the Huh-7 cell xenograft mouse model and found that the tumor growth was significantly suppressed when inoculated mice were injected with AdFOXM1shRNA in the tumors. Together, our results suggest that FOXM1 is a potential therapeutic target for HCC and AdFOXM1shRNA may be an additional gene therapeutic intervention for HCC treatment.
Insights
Forkhead Box M1 (FOXM1) is crucial for liver cancer growth. Targeting FOXM1 with gene therapy (AdFOXM1shRNA) effectively suppressed hepatocellular carcinoma (HCC) cell proliferation and tumor growth in mice.
Area of Science:
- Oncology
- Molecular Biology
- Gene Therapy
Background:
- The Forkhead Box M1 (FOXM1) transcription factor plays a significant role in human carcinomas.
- FOXM1 regulates genes involved in cell proliferation and transformation, making it a potential therapeutic target.
Purpose of the Study:
- To investigate the role of FOXM1 in hepatocellular carcinoma (HCC).
- To evaluate the efficacy of AdFOXM1shRNA as a gene therapeutic intervention for HCC.
Main Methods:
- FOXM1 expression was analyzed in clinical HCC tissues and cell lines (Huh-6, Huh-7, HepG2).
- FOXM1 knockdown was achieved using an adenovirus vector expressing short hairpin RNA (AdFOXM1shRNA).
- Cell proliferation, anchorage-independent growth, and tumor xenograft growth in mice were assessed.
Main Results:
- FOXM1 was highly expressed in HCC specimens and cell lines.
- AdFOXM1shRNA significantly reduced proliferation of Huh-7 and HepG2 cells and anchorage-independent growth of Huh-7 cells.
- AdFOXM1shRNA injection suppressed tumor growth in a Huh-7 cell xenograft mouse model.
Conclusions:
- FOXM1 is a promising therapeutic target for HCC.
- AdFOXM1shRNA demonstrates potential as a gene therapeutic strategy for HCC treatment.
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