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Expression of domains of mouse alpha-fetoprotein in Escherichia coli
R Boismenu1, M S Dubow, R A Murgita
1Department of Microbiology and Immunology, McGill University, Montreal, Canada.
Life Sciences
|January 1, 1988
Abstract:
A mouse alpha-fetoprotein complementary DNA containing the coding region for amino acids 256 to 548 was fused to the lac transcriptional and translational control elements contained on the expression vector pOP203-28. The expression of a 35 kD hybrid lac Z'- alpha-fetoprotein polypeptide in Escherichia coli was demonstrated by the chloramphenicol release assay and by immunoprecipitation using rabbit anti-mouse alpha-fetoprotein antibodies as probe.