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Metachromatic procedure for fiber typing in chicken skeletal muscle
1Muscle Biology Laboratory, University of Wisconsin, Madison 53706.
Poultry Science
|June 1, 1988
Abstract:
Metachromatic staining of anterior latissimus dorsi, posterior latissimus dorsi, and sartorius muscles of domestic chicken with azure A and toluidine blue produced differentiation of muscle fiber types. The procedure is rapid, with optimum results obtained after 2 to 5 min incubation and 5 to 10 s of staining. Reaction of serial sections for myosin Ca++-adenosine triphosphatase with the usual procedure showed that the same fibers were typed similarly with both methods.