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Updated: May 2, 2026
Particle Agglutination Method for Poliovirus Identification
Published on: April 20, 2011
[Identification and characterization of enterovirus 71 isolated in Yunnan province]
Shi-Wen Zhao1, Wei-Dong Yin1, Jian-Ping Cun1
1Yunnan Center for Disease Control, Kunming 650022, China.
Objective:
Identification and characterization of eight enterovirus 71 samples isolated from Yunnan province.
Methods:
Collecting specimens from cases of Hand, Foot and Mouth Disease (HFMD), samples were identificated by RT-PCR, isolated the virus with cells. The isolating virus passaged in vero cells, and the vpl genes sequencing. Immunization the mice with the virus, neutralization test and cross-protection test were conducted with those antisera.
Result:
All samples were identified as EV71, and eight strains had cytopathic effect (CPE) in vero cells, some antisera titers were more than 1:10,000.
Conclusion:
Eight strains of the virus grow well in vero cell, antiserum showed protection against EV71 A and C4 genotype.
