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[Construction of recombinant CHO cell strain for high expression of HBsAg]
Yao Yi1, Min-Zhuo Guo1, Rui-Guang Tian1
1National Institute for Viral Disease Contral and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
Objective:
To overexpress hepatitis B virus S gene in CHO cells cultured in serum-free media.
Method:
Plasmid was constructed by cloning of HBV S gene and then it was transfected into CHO cells. After cell screen, the positive clones were identified and isolated into a serum-free media followed by the serological and morphological characterization of the expression product.
Result:
CHO cell strains which can express HBsAg efficiently and stably were obtained. Spherical and filamentous HBsAg could be detected under electronic microscope. The titer of the expression product was up to 1:5000.
Conclusion:
Serum-free media cultured CHO cell strain for overexpression of HBsAg was successfully constructed and the expression product was high antigenic.
