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[Establishment of a detection method for hepatitis B virus large surface protein (HBV-lP)]
Jing Zhao1, Hong-Bin Ma1, Jun Hou1
1Center of Clinical Laboratory, the No. 302 Hospital of the PLA, Beijing 100039, China.
Objective:
To establish enzyme-linked immunosorbent assay (ELISA) for detection of hepatitis B virus large surface protein(HBV-LP) in serum.
Methods:
A sandwich reaction was preformed with horseradish peroxidase labeled monoclonal antibody of HBV-LP as the catalytic enzyme. Several reactions liquid's concentration and reaction conditions were optimized. The method was evaluated in all aspects such as sensitivity, specificity, stability and so on.
Results:
The detection limit was 5 ng/ml. Interassay and intra-assay RSD were both less than 10%. After stored at 4 degrees C and 37 degrees C for 3, 5, 7 days, the analysis showed correlation coefficient higher than 0.98 and RSD lower than 10%.
Conclusion:
Established ELISA for determination of serum HBV-LP has high sensitivity and repeatability. Enzyme-linked immunosorbent assay;
