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Stretch-activated cation channels in human fibroblasts.

L L Stockbridge1, A S French

  • 1Department of Physiology, University of Alberta, Edmonton, Canada.

Biophysical Journal
|July 1, 1988
PubMed
Summary

Human fibroblasts exhibit stretch-activated cation channels responsible for electrical activity. These channels, distinct from calcium-activated potassium channels, adapt to sustained stretch but maintain a static component, influencing cell behavior.

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Area of Science:

  • Cell biology
  • Biophysics
  • Physiology

Background:

  • Fibroblasts exhibit electrical activity, including transient hyperpolarizations, influenced by external stimuli and cellular states.
  • Confluence in fibroblasts leads to cessation of electrical, growth, and mitogenic activity.
  • A calcium-activated potassium conductance was previously hypothesized for fibroblast hyperpolarizations, but not found to be stretch-activated in human cells.

Purpose of the Study:

  • To identify stretch-activated ion channels in human fibroblasts.
  • To characterize the properties of these stretch-activated channels.

Main Methods:

  • Utilized cell-attached and inside-out patch clamp techniques.
  • Investigated single channel activity in response to mechanical stretch.

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Main Results:

  • Identified single stretch-activated cation channels in human fibroblasts.
  • The predominant channel exhibited a conductance of approximately 60 pS (picoSiemens) in 140 mM potassium, permeable to both potassium and sodium ions.
  • Observed significant adaptation of channel activity under sustained stretch, with a persistent static component.
  • Detected higher conductance channels in some excised patches.

Conclusions:

  • Human fibroblasts possess stretch-activated cation channels, distinct from previously studied calcium-activated potassium channels.
  • These channels contribute to the electrical activity of fibroblasts and adapt to mechanical stimuli.
  • The identified channels may play a role in the regulation of fibroblast behavior in response to mechanical cues.