Yeast two-hybrid screen
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A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions
Published on: June 6, 2018
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1Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Methods in Enzymology
|March 4, 2014
Abstract:
Yeast two-hybrid is a method for screening large numbers of gene products (encoded by cDNA libraries) for their ability to interact with a protein of interest. This system can also be used for characterizing and manipulating candidate protein: protein interactions. Interactions between proteins are monitored by the growth of yeast plated on selective media.
Keywords:
Activation domain (AD) fusion proteinBack-transformation of yeastCloning and transformation methodsConfirmation of positive interactorsDNA-binding domain (DB) fusion proteinElectroporation of E. coliPlasmid rescueSelf-activation of pDBLeu-X (and pPC86-Y)Small-scale yeast transformationYeast two-hybrid screenpDBLeu-X
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