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Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution
Published on: January 16, 2017
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Analysis of RNA-protein interactions by cell mixing
Sarah H Stubbs1, Nicholas K Conrad1
1Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, TX, USA.
Methods in Enzymology
|March 4, 2014
Abstract:
RNA-protein complexes are critical for almost all aspects of gene expression. Analysis of RNA-protein interactions can be complicated by the disruption of native complexes and the formation of new, reassorted complexes upon cell lysis. Before concluding that a specific RNA and protein interact in vivo, cell-mixing experiments can be performed to ensure that observed RNA-protein complexes are not formed after lysis of cells.

