Identifying proteins that bind a known RNA sequence using the yeast three-hybrid system

Related Experiment Video

Updated: May 2, 2026

Novel RNA-Binding Proteins Isolation by the RaPID Methodology
11:19

Novel RNA-Binding Proteins Isolation by the RaPID Methodology

Published on: September 30, 2016

8.8K

Yvonne Y Koh1, Marvin Wickens2

  • 1Microbiology Doctoral Training Program, University of Wisconsin - Madison, Madison, WI, USA.

Methods in Enzymology
|March 4, 2014
PubMed

Keywords:
3-Aminotriazole (3-AT) concentrationElimination of RNA-independent false positivesRNA plasmidTransformation efficiencyYeast three-hybrid systemcDNA libraryβ-Galactosidase activity

More Related Videos

Enhanced Yeast One-hybrid Screens To Identify Transcription Factor Binding To Human DNA Sequences11:25

Enhanced Yeast One-hybrid Screens To Identify Transcription Factor Binding To Human DNA Sequences

Published on: February 11, 2019

7.7K
A Modified Yeast-one Hybrid System for Heteromeric Protein Complex-DNA Interaction Studies10:47

A Modified Yeast-one Hybrid System for Heteromeric Protein Complex-DNA Interaction Studies

Published on: July 24, 2017

10.6K

Related Concept Videos

Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.2K