Identification of an antigenic determinant in the amino-terminal sequence of parathyroid hormone

The application of a hydrophilicity plot to define an antigenic determinant in the amino-terminal sequence of human parathyroid hormone is reported. The data obtained were compared to specificity studies of three antibodies, one polyclonal and two monoclonal, against the same portion of the molecule. Results were coincident. These data support the use of the hydrophilicity plot in the prediction of antigenic sites in the parathyroid hormone molecule.