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Updated: May 2, 2026

Detection of Live Escherichia coli O157:H7 Cells by PMA-qPCR
Published on: February 1, 2014
Improved PCR-RFLP method for the identification of Escherichia coli enterohemolysin (ehxA) subtypes
Sandra C Lorenz1, Markus Fischer2, Julie A Kase3
1U.S. Food and Drug Administration, Center for Food Safety and Applied Nutrition, Division of Microbiology, College Park, MD 20740, USA; University of Hamburg, Hamburg School of Food Science, Institute of Food Chemistry, Grindelallee 117, 20146 Hamburg, Germany.
Abstract:
Previously published PCR-RFLP methods failed to detect the six genetically different Escherichia coli ehxA subtypes reliably. Here we describe an improved PCR-RFLP method that detects all ehxA subtypes. Importantly, ehxA-subtyping may contribute to the detection of potentially pathogenic STECs and can expedite initial screenings of patient samples and food enrichments.
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