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Related Experiment Videos

A scanning ocular spectrofluorophotometer.

J W McLaren1, R F Brubaker

  • 1Department of Ophthalmology, Mayo Clinic, Rochester, MN 55905.

Investigative Ophthalmology & Visual Science
|August 1, 1988
PubMed
Summary

A new scanning ocular spectrofluorophotometer (SOSF) enables in-vivo measurement of ocular fluorescence. This instrument tracks fluorophore distribution and kinetics in the anterior eye, advancing ophthalmic research.

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Area of Science:

  • Ophthalmology
  • Biomedical Engineering
  • Spectroscopy

Background:

  • Ocular drug delivery and pharmacokinetics require precise measurement techniques.
  • Existing methods for in-vivo ocular fluorescence measurement are limited.

Purpose of the Study:

  • To introduce and validate a novel Scanning Ocular Spectrofluorophotometer (SOSF).
  • To demonstrate the SOSF's capability in measuring fluorescence and kinetics within the anterior eye.

Main Methods:

  • Developed a Scanning Ocular Spectrofluorophotometer (SOSF) with rapid wavelength switching.
  • Utilized a diffraction grating monochromator and variable interference filters for excitation and emission.
  • Administered fluorescein, fluorescein glucuronide, rhodamine B, and fluorescein-labeled dextran topically and intracamerally in rabbit eyes.

Main Results:

  • Successfully measured fluorescence spectra of various fluorophores in rabbit anterior chamber and cornea.
  • Observed rapid corneal and lens absorption of rhodamine B.
  • Found fluorescein-dextran was not measurable in the cornea until 4 hours post-injection.

Conclusions:

  • The SOSF is a versatile tool for in-vivo spectrofluorophotometry of the living eye.
  • The instrument facilitates kinetic studies of ocular drug distribution and clearance.
  • SOSF measurements provide valuable data for ophthalmic research and drug development.

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