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Bombyx mori nucleopolyhedrovirus ORF79 is a per os infectivity factor associated with the PIF complex

Zhan-Qi Dong1, Jun Zhang1, Xue-Mei Chen1

  • 1State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China.

Virus Research
|March 4, 2014
PubMed

Insights

Bombyx mori nucleopolyhedrovirus (BmNPV) ORF79 (Bm79) is crucial for oral infection. Deleting Bm79 eliminated per os infectivity, highlighting its role in the Bombyx mori nucleopolyhedrovirus life cycle and PIF complex.

Area of Science:

  • Virology
  • Molecular Biology
  • Insect Pathology

Background:

  • Bombyx mori nucleopolyhedrovirus (BmNPV) is a significant pathogen affecting silkworms.
  • Understanding viral infection mechanisms is key to developing control strategies.

Purpose of the Study:

  • To investigate the function of BmNPV ORF79 (Bm79) in the viral life cycle.
  • To determine the role of Bm79 in per os infection.

Main Methods:

  • Construction of a Bm79 knockout virus (vBm(Bm79KO)) using homologous recombination.
  • Analysis of viral DNA replication, budded virus (BV) production, and polyhedra formation.
  • Larval bioassays and immunofluorescence analysis to assess infectivity and protein localization.
  • Bimolecular fluorescence protein complementation and co-immunoprecipitation to study protein interactions.

Main Results:

  • Viral DNA replication, BV production, and polyhedra formation were not affected by Bm79 deletion.
  • Bm79 knockout resulted in a complete loss of per os infectivity in larvae.
  • Bm79 localized to the inner nuclear membrane via its N-terminal sorting motif.
  • Bm79 interacts with PIF1, PIF2, PIF3, and ODV-E66, forming part of the PIF complex.

Conclusions:

  • Bm79 is essential for per os infection of BmNPV.
  • Bm79 is a component of the viral PIF complex, crucial for oral infectivity.
Keywords:
Bm79BmNPVBombyx moriPIF complexPer os infection

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