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Updated: May 2, 2026
A Polyaniline-based Sensor of Nucleic Acids
Published on: November 1, 2016
A novel label-free amperometric immunosensor for carcinoembryonic antigen based on Ag nanoparticle decorated infinite
Wenbo Lu1, Xiaowei Cao1, Lin Tao1
1State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, PR China.
Abstract:
In this article, for the first time, a novel, high-yield and template-free method for the synthesis of Ag nanoparticle decorated thionine/infinite coordination polymer (AgNP/THI/ICP) fibres is proposed. The thionine can be adsorbed to the AgNP/THI/ICP fibres by π-conjugation and act as the redox probe. The AgNP/THI/ICP fibres not only favor the immobilization of antibody but also facilitate the electron transfer. It is found that the AgNP/THI/ICP fibres can be designed to act as a sensitive label-free electrochemical immunosensor for carcinoembryonic antigen (CEA) determination. Under the optimized conditions, the linear range of the proposed immunosensor is estimated to be from 50 fg/mL to 100 ng/mL and the detection limit is estimated to be 0.5 fg/mL at a signal-to-noise ratio of 3, respectively. The prepared immunosensor for detection of CEA shows high sensitivity, reproducibility and stability. Our study demonstrates that the proposed immunosensor has also been used to determine CEA successfully in diluted blood samples.
