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Updated: May 2, 2026

A Flexible Chamber for Time-Lapse Live-Cell Imaging with Stimulated Raman Scattering Microscopy
Published on: August 31, 2022
Live-cell imaging of alkyne-tagged small biomolecules by stimulated Raman scattering
Lu Wei1, Fanghao Hu1, Yihui Shen1
1Department of Chemistry, Columbia University, New York, New York, USA.
Abstract:
Sensitive and specific visualization of small biomolecules in living systems is highly challenging. We report stimulated Raman-scattering imaging of alkyne tags as a general strategy for studying a broad spectrum of small biomolecules in live cells and animals. We demonstrate this technique by tracking alkyne-bearing drugs in mouse tissues and visualizing de novo synthesis of DNA, RNA, proteins, phospholipids and triglycerides through metabolic incorporation of alkyne-tagged small precursors.

