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Multigene phylogeny, taxonomy and reclassification of Hyaloperonospora on Cardamine

Hermann Voglmayr1, Young-Joon Choi2, Hyeon-Dong Shin3

  • 1Department of Systematic and Evolutionary Botany, Faculty Center Biodiversity, University of Vienna, Rennweg 14, 1030 Wien, Austria. hermann.voglmayr@univie.ac.at.

Mycological Progress
|March 4, 2014
PubMed

Insights

Six species of Hyaloperonospora, a plant pathogen, were identified on Cardamine plants using DNA sequencing. These species, previously misclassified, are now clarified, with one new species described. Host specificity is key for identification.

Area of Science:

  • Mycology
  • Plant Pathology
  • Molecular Systematics

Background:

  • The genus Hyaloperonospora comprises downy mildews, important plant pathogens.
  • Previous classifications often grouped distinct Hyaloperonospora species under Peronospora dentariae.
  • Morphological characteristics alone are insufficient for accurate species delimitation within Hyaloperonospora on Cardamine.

Purpose of the Study:

  • To resolve the species diversity of Hyaloperonospora on the genus Cardamine.
  • To clarify the taxonomic circumscription of several Hyaloperonospora species.
  • To evaluate the utility of specific genetic markers for species delimitation.

Main Methods:

  • Phylogenetic analysis using sequence data from mitochondrial genes (cox1, cox2) and ribosomal DNA (ITS, LSU).
  • Comparative analysis of type specimens and host range data.
  • Morphological examination of specimens.

Main Results:

  • At least six distinct Hyaloperonospora species were identified on Cardamine.
  • Five species, including Peronospora dentariae, were combined under Hyaloperonospora, with clarified taxonomic boundaries.
  • A new species, Hyaloperonospora cardamines-enneaphyllos, was described.
  • Hyaloperonospora nasturtii-aquatici demonstrated an expanded host range to include various Cardamine species.
  • Host specificity proved to be a reliable diagnostic character, with no overlapping host ranges observed among the studied species.

Conclusions:

  • Molecular data, particularly cox1 and cox2 sequences, are effective for distinguishing closely related Hyaloperonospora species on Cardamine.
  • Host range is a crucial, albeit often overlooked, diagnostic feature for Hyaloperonospora species identification.
  • Accurate species identification within Hyaloperonospora requires a combination of molecular and host range data, as morphology is often ambiguous.

Keywords:
DentariaHost rangeObligate parasitesPeronosporaPeronosporaceae

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