Related Experiment Video
Updated: May 2, 2026

Isolation of Soil Microorganisms Using iChip Technology
Published on: January 10, 2025
Microbial community structures of novel Icelandic hot spring systems revealed by PhyloChip G3 analysis
Jordan E Krebs1, Parag Vaishampayan, Alexander J Probst
11 Biotechnology and Planetary Protection Group, Jet Propulsion Laboratory , California Institute of Technology, Pasadena, California, USA.
Abstract:
Microbial community profiles of recently formed hot spring systems ranging in temperatures from 57°C to 100°C and pH values from 2 to 4 in Hveragerði (Iceland) were analyzed with PhyloChip G3 technology. In total, 1173 bacterial operational taxonomic units (OTUs) spanning 576 subfamilies and 38 archaeal OTUs covering 32 subfamilies were observed. As expected, the hyperthermophilic (∼100°C) spring system exhibited both low microbial biomass and diversity when compared to thermophilic (∼ 60°C) springs. Ordination analysis revealed distinct bacterial and archaeal diversity in geographically distinct hot springs. Slight variations in temperature (from 57°C to 64°C) within the interconnected pools led to a marked fluctuation in microbial abundance and diversity. Correlation and PERMANOVA tests provided evidence that temperature was the key environmental factor responsible for microbial community dynamics, while pH, H2S, and SO2 influenced the abundance of specific microbial groups. When archaeal community composition was analyzed, the majority of detected OTUs correlated negatively with temperature, and few correlated positively with pH.
More Related Videos
11:07In Situ Isolation and Culturing of Recalcitrant Soil Bacteria using an Isolation Chip (iChip)
Published on: August 6, 2025
08:09An Aquatic Microbial Metaproteomics Workflow: From Cells to Tryptic Peptides Suitable for Tandem Mass Spectrometry-based Analysis
Published on: September 15, 2015
Related Concept Videos
iChip
Microbial Mats
Methods to Assess Microbial Communities
Hyperthermophilic Bacteria
Viruses of Archaea
Modern Molecular Taxonomy