Synthesis of capped RNA using a DMT group as a purification handle
Elizabeth Veliath1, Barbara L Gaffney, Roger A Jones
1a Department of Chemistry and Chemical Biology, Rutgers , The State University of New Jersey , Piscataway , New Jersey , USA.
Nucleosides, Nucleotides & Nucleic Acids
|March 5, 2014
Abstract:
We report a new method for synthesis of capped RNA or 2'-OMe RNA that uses a N(2-)4,4'-dimethoxytrityl (DMT) group as a lipophilic purification handle to allow convenient isolation and purification of the capped RNA. The DMT group is easily removed under mild conditions without degradation of the cap. We have used this approach to prepare capped 10- and 20-mers. This method is compatible with the many condensation reactions that have been reported for preparation of capped RNA or cap analogues.
