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Related Concept Videos

Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

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Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
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High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging
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Color reflectance fiber bundle endomicroscopy without back-reflections.

Michael Hughes, Petros Giataganas, Guang-Zhong Yang

    Journal of Biomedical Optics
    |March 5, 2014
    PubMed
    Summary

    This study presents a novel method for widefield endomicroscopy using fiber imaging bundles. The technique injects light near the fiber

    Area of Science:

    • Biomedical Optics
    • Medical Imaging
    • Fiber Optics

    Background:

    • Coherent fiber imaging bundles are suitable for passive reflectance-mode endomicroscopy.
    • Efficient rejection of back-reflections from fiber ends is crucial for effective imaging.

    Purpose of the Study:

    • To develop and demonstrate a method for widefield endomicroscopy that minimizes artifact-causing back-reflections.
    • To enable color widefield reflectance imaging of biological tissues using fiber bundles.

    Main Methods:

    • A novel approach for widefield endomicroscopy involving light injection near the distal end of a leached fiber bundle.
    • This method effectively bypasses reflections from the proximal fiber face.

    Main Results:

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  • Successful demonstration of color widefield reflectance endomicroscopy.
  • Imaging was performed on ex vivo animal tissue, showcasing the technique's applicability.

    • Conclusions:

    • The described light injection method overcomes limitations of traditional fiber bundle endomicroscopy.
    • This technique facilitates high-quality, artifact-reduced color widefield reflectance imaging of tissues.